Protein on nitrocellulose sheet support

Details Protein on nitrocellulose sheet support Protein on nitrocellulose sheet support

G - Physics

01

N

167/37, 204/98.0

G01N 27/26 (2006.01) G01N 27/447 (2006.01) G01N 33/558 (2006.01)

Patent

CA 1152430

Abstract of the Disclosure The invention concerns new solid supports for proteins con- sisting of nitrocellulose sheets containing a replica of an electro- pherogram of proteins as obtained by electrophoretic separation in a gel. Faithful replica of such electropherograms on the nitrocellulose support can be obtained by contacting the gel with a nitrocellulose sheet and applying an electric field perpendicular to the plans of the gel causing an electrophoratic migration of the proteins toward the nitrocellulose sheet where the proteins are adsorbed. The process is easier to perform than similar procedures described in the art. Various analytical problems and especially immuno-assays, for instance enzyme immuno-assays or such involving radioactively labeled indicators, can be made with the new solid supports.

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