Protein partner screening assays and uses thereof

Details Protein partner screening assays and uses thereof Protein partner screening assays and uses thereof

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C07K 14/01 (2006.01) C07K 14/82 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2077439

2077439 9116429 PCTABS00008 A rapid, simple and inexpensive method to screen and classify proteins as partners of heterodimeric proteins is described which utilizes fusion protein constructs containing a DNA binding domain and a dimerization domain from a different protein. According to the method of the invention, heterodimer formation is detected by the ability of the protein partner to displace homodimer formation, and thus reveal a phenotypic change in a bacterial host which was dependent upon maintenance of the homodimer configuration. The method of the invention may be used to identify compounds of interest which inhibit such heterodimer formation, and especially to identify compounds which prevent heterodimer formation and activation of oncogenic transcriptional regulatory proteins.

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