C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
167/113, 530/9,
C07K 16/18 (2006.01) C07K 14/47 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)
Patent
CA 1234100
- 1 - Abstract of the disclosure The protein PP20 is described, this having the following characteristics: a) an electrophoretic mobility which is somewhat larger than that of albumin, b) an isoelectric point of 4.65 ? 0.1, c) a sedimentation coefficient s20,w of 4.1 ? 0.1 S, d) a molecular weight determined by electrophoresis in sodium dodecyl sulfate (SDS)-containing polyacrylamide gel of 50,000 ? 10,000, the molecules of PP20 being composed of apparently identical subunits which have a molecular wright of 27,000 ? 3,000 and are held together non-covalently, e) an extinction coefficient Image (280 nm) of 9.5 + 0.6, f) a carbohydrate content of 3.0 ? 1.3%, including mannose 0.14 ? 0.05%, fucose 0.13 ? 0.05%, galactose 0.61 ? 0.2%, glucose 0.39 ? 0.2%, N- acetylglucosamine 0.95 ? 0.3%, N-acetylgalactosamine 0.18 ? 0.1%, and N-acetylneuraminic acid 0.6 ? 0.4 %. g) a specified aminoacid composition, and a process for obtaining it is described. This protein can be used to obtain antisera and to design immunochemical methods which can be used to detect and determine this protein, in order to monitor pregnancy or to detect or check the course of diseases.
465968
Bohn Hans
Winckler Wilhelm
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Dade Behring Marburg Gmbh
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