Protein production at synthetic start site

Details Protein production at synthetic start site Protein production at synthetic start site

C - Chemistry, Metallurgy

12

N

195/86, 195/1.2,

C12N 15/00 (2006.01) C12N 9/06 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1193208

ABSTRACT OF THE DISCLOSURE Method and compositions are provided for pro- ducing proteins expressed by a DNA sequence free of proteins expressed by other DNA sequences where the DNA sequences comprise a functional recombinant DNA struc- ture. The functional recombinant DNA structure is formed by annealing and/or ligating a first DNA sequence from a first source which encodes for a polypeptide; a second DNA sequence comprising a vector compatible with a unicellular organism which does not exchange genetic information with said first source, said vector having an intact replicon and a promoter for said first DNA sequence; and a third DNA sequence synthetically intro- duced between said first and second DNA sequences either prior to or concomitantly with annealing and/or ligating of said first and second DNA sequences encoding for a ribosomal binding site of said host, said third DNA sequence positioned adjacent a deoxyribonucleotide triplet encoding for methionine. The recombinant DNA structure is then employed in the transformation of a unicellular host to provide the host the capability of producing the polypeptide free of other polypeptides.

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