Protein production using eukaryotic cell lines

Details Protein production using eukaryotic cell lines Protein production using eukaryotic cell lines

C - Chemistry, Metallurgy

12

N

C12N 15/79 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2654415

The subject invention provides a site-specific integration system and methods for generating eukaryotic cells lines for protein production. The provided system includes a first site-specifically integrating target vector and a second site- specifically integrating donor vector comprising a gene of interest. Also provided are mammalian cell lines produced by the subject methods and systems, as well as kits that include the subject systems.

La présente invention concerne un système d'intégration spécifique d'un site et des procédés destinés à générer des lignées de cellules eucaryotes pour la production de protéines. Le système élaboré inclut un premier vecteur cible s'intégrant spécifiquement au site et un second vecteur donneur s'intégrant spécifiquement au site et comportant un gène d'intérêt. L'invention concerne également des lignées cellulaires mammaliennes produites par les présents procédés et systèmes, ainsi que des trousses qui incluent lesdits systèmes.

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