Protein purification

Details Protein purification Protein purification

C - Chemistry, Metallurgy

07

K

530/15.14, 530/3

C07K 14/505 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1297635

- 18 - "PROTEIN PURIFICATION" ABSTRACT Chromatographic procedures are individually and jointly applied to the rapid and efficient isolation of biologically active proteins and especially glycopro- teins such as recombinant erythropoietin present in the medium of growth of genetically transformed mammalian host cells. Illustratively, recombinant EPO is isolated from culture fluids by reverse phase chromatography employing a C4 or C6 column and elution with ethanol. Recombinant erythropoietin may also be purified by anion exchange chromatography employing, e.g., a DEAE resin, with preliminary selective elution of contaminant materials having a lower pKa than erythropoietion from the resin under conditions mitigating against acid ac- tivated protease degradation. Practiced serially, the two chromatographic procedures allow for high yields of biologically active recombinant erythropoietin from mammalian cell culture media.

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