C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) A61K 39/395 (2006.01) C07K 1/18 (2006.01) C07K 16/06 (2006.01) C07K 16/24 (2006.01)
Patent
CA 2042945
Method of purifying a protein from a solution of substances comprising the steps of passing the solution through an ion exchange material at a pH which facilitates binding of the protein of interest, washing the bound protein at a different pH at which the bound protein does not elute but at which the free protein could not bind to the ion exchange material, and then eluting the protein at a pH which facilitates elution. Method is especially useful in purification of antibodies such as antibodies to tumor necrosis factor.
Coan Michael H.
Lee Vivian W.
Bayer Pharmaceuticals Corporation
Miles Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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