Protein recovery

Details Protein recovery Protein recovery

C - Chemistry, Metallurgy

12

P

530/3.2

C12P 21/02 (2006.01) C07K 1/113 (2006.01) C07K 14/61 (2006.01)

Patent

CA 1267497

ABSTRACT OF THE DISCLOSURE An improved process is described for recovering proteins from insoluble inclusion bodies produced in transformant microorganisms. Sidestream precipitates isolated from chromatography effluent are resolubilized in a denaturing agent and the resolubilized proteins are renatured either by direct dialysis into denaturant-free buffer or by dialysis into a weaker denaturing agent followed by dialysis into denaturant-free buffer.

517714

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