Protein recovery by chromatography followed by filtration...

Details Protein recovery by chromatography followed by filtration... Protein recovery by chromatography followed by filtration...

C - Chemistry, Metallurgy

07

K

C07K 1/36 (2006.01) C07K 16/06 (2006.01)

Patent

CA 2289665

The invention herein provides a method for recovering a polypeptide comprising exposing a composition comprising a polypeptide to a reagent which binds to, or modifies, the polypeptide, wherein the reagent is immobilized on a solid phase; and then passing the composition through a filter bearing a charge which is opposite to the charge of the reagent in the composition, so as to remove leached reagent from the composition.

La présente invention concerne un procédé de récupération d'un polypeptide consistant à exposer une composition comprenant un polypeptide, à un réactif qui lie ou modifie ledit polypeptide, où le réactif est immobilisé en phase solide ; puis à faire passer la composition à travers un filtre portant une charge opposée à la charge du réactif de la composition de façon à éliminer le réactif lixivié de la composition.

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