C - Chemistry – Metallurgy – 09 – B
Patent
C - Chemistry, Metallurgy
09
B
C09B 53/00 (2006.01) C09B 1/34 (2006.01) C09B 1/36 (2006.01) C09B 11/10 (2006.01) C09B 57/00 (2006.01) C09B 69/06 (2006.01) G01N 27/447 (2006.01) G01N 33/52 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2096542
2096542 9208689 PCTABS00013 The present invention provides novel dyes for staining proteins, particularly those in electrophoretic gels, and detecting the proteins with high sensitivity. The dyes of the invention are derivatives of known dyes that are more hydrophobic than the corresponding, known dyes and, in eletrophoretic gels, including polyacrylamide gels with sodium dodecyl sulfate, form easily detectable, non-vovalent complexes with proteins. In some cases, the corresponding, known dyes bind to protein covalently. The complexes of protein with some dyes of the invention can be detected with high sensitivity simply by observation of the color of the complexes by eye. The complexes of protein with other dyes of the invention are detected by observation of the fluorescence of the complexes. Dyes of the invention include derivatives of CoomassieR dyes, acid blue 25, dansyl chloride, fluorescein, and 2-methyl-2,4-diphenyl-3(2H)-furanone. The invention also provides quantitative and qualitative methods for detecting proteins which comprise staining the proteins with a dye of the invention.
Leland David L.
Shultz John W.
Borden Ladner Gervais Llp
Promega Corporation
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