Protein tag comprising a biotinylation domain and method for...

Details Protein tag comprising a biotinylation domain and method for... Protein tag comprising a biotinylation domain and method for...

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C12N 9/00 (2006.01) C12N 15/10 (2006.01) C12N 15/67 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2474457

The use of a tag moiety comprising a biotinylation domain, such as biotin carboxyl carrier protein (BCCP), as a protein folding marker and protein solubility enhancer in the orientated surface capture of products of heterologously expressed genes is described. Methods for increasing the solubility of proteins and determining the folded state of a protein are also disclosed. The uses and methods of the invention can be carried out in a multiplexed manner on more than one protein in the formation of libraries. In addition the nucleic acid molecule encoding the biotinylation domain of the tag moiety can be used to increase the proportion of clones in a library that express the protein of interest.

L'invention concerne l'utilisation d'une fraction étiquette comprenant un domaine de biotinylation, telle qu'une protéine porteuse de carboxyle de biotine (BCCP), en tant que marqueur de pliage de protéine et solubilisant de protéine dans la capture de surface orientée de produits de gènes exprimés de façon hétérologue. L'invention concerne également des procédés d'augmentation de la solubilité de protéines et de détermination de l'état plié d'une protéine. Les utilisations et procédés selon l'invention peuvent être mis en oeuvre de façon multiplexée sur plus qu'une protéine, lors de la formation de banques. En outre, la molécule d'acide nucléique codant le domaine de biotinylation de la fraction étiquette peut être utilisée pour l'augmentation de la proportion de clones dans une banque qui expriment la protéine considérée.

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