C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12P 1/00 (2006.01) C12P 7/64 (2006.01) C07K 14/195 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C12N 9/00 (2006.01)
Patent
CA 2655764
The present invention relates to a method of targeting a protein of interest to an intracellular hydrophobic inclusion body of a bacterial cell by means of a fusion protein comprising a hydrophobic targeting peptide operatively linked with said protein of interest; methods of microbial production of a lipophilic compound of interest by means of a recombinant bacterial host comprising intracellular inclusion bodies having at least one enzyme which is involved in the biosynthesis of said lipophilic compound targeted to said inclusion bodies; as well as corresponding fusion proteins, coding sequences, expression vectors and recombinant hosts.
La présente invention concerne un procédé de ciblage d'une protéine intéressante pour un corps d'inclusion intracellulaire hydrophobe d'une cellule bactérienne à l'aide d'une protéine de fusion, comprenant un peptide hydrophobe de ciblage, lié de manière fonctionnelle à ladite protéine intéressante ; des procédés de production microbienne d'un composé lipophile intéressant à l'aide d'un hôte bactérien recombiné comprenant des corps d'inclusion intracellulaire, présentant au moins une enzyme qui est impliquée dans la biosynthèse dudit composé lipophile ciblé sur les corps d'inclusion ; ainsi que les protéines de fusion, les séquences codantes, les vecteurs d'expression et les hôtes recombinés correspondants.
Haenisch Jan
Steinbuechel Alexander
Waelterman Marc
Basf Se
Robic
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