C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/33
C12P 21/00 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1128440
ABSTRACT OF THE INVENTION An improved method for in vitro synthesis of protein by in vivo processes in an extract from a living organism and contain- ing endogenous ribosomes and endogenous transfer RNA, primarily from endogenous amino acids in said extract primarily by the use of a predetermined exogenous messenger ribonucleic acid (mRNA). An improved method of assaying such a syntheses by the use of a labelled amino acid, which is present during said synthesis and is incorporated into into said protein. A kit for carrying out such synthesis and assay compris- ing a vial of substantially prokaryotic and eukaryotic cell-free extract from a living organism containing endogenous ribosomes, endogenous transfer RNA and endogenous amino acid, a vial contain- ing translation cocktail for promoting the synthesis, and a vial containing control messenger RNA.
339088
Nen Life Science Products Inc.
Sherman
LandOfFree
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