Protein translocation into plant cells

Details Protein translocation into plant cells Protein translocation into plant cells

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/195 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2409236

The invention relates to a method of effecting a change in a cell using a transfer system which is contacted with the cell to be changed. The transfer system comprises a fusion protein which is transferred into the cell using the VirB/VirD4 transfer system. According to the invention a fusion protein BA is introduced into the cell to be changed comprising i) as a first part A an oligopeptide comprising 3 adjacent amino acids identical to or corresponding to the amino acids of the C-termninal amino acids 1-40 of VirF, VirD2, VirE2, VirE3 or MobA, and ii) as a second part B a polypeptide capable of effecting a cell-changing activity in the cell to be changed, wherein the polypeptide is attached with the C-terminal end thereof with the N-terminal end of the first part.

L'invention concerne un procédé destiné à provoquer une modification dans une cellule au moyen d'un système de transfert, lequel est mis en contact avec la cellule à modifier. Le système de transfert comprend une protéine hybride, laquelle est transférée dans la cellule au moyen du système de transfert VirB/VirD4. Selon l'invention, une protéine hybride BA est introduite dans la cellule à modifier. Cette protéine comprend i) comme première partie A un oligopeptide contenant 3 acides aminés adjacents identiques ou correspondant aux acides aminés 1-40 C-terminaux de VirF, VirD2, VirE2, VirE3 ou MobA, et ii) comme seconde partie B un polypeptide capable de provoquer une modification de l'activité cellulaire dans la cellule à modifier, le polypeptide étant relié avec son extrémité C-terminale à l'extrémité N-terminale de la première partie. (Fig.).

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