C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A61K 38/45 (2006.01) C07K 14/705 (2006.01) C07K 14/71 (2006.01) C07K 14/715 (2006.01) C07K 16/28 (2006.01) C07K 16/40 (2006.01) C12N 9/12 (2006.01) C12Q 1/48 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/573 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2175893
The receptor protein tyrosine kinase (rPTK) designed Rse has been identified from human and murine cell tissues. DNA encoding Rse rPTK has been cloned from a cDNA library of a human liver carcinoma cell Line (i.e., Hep 3B) using PCR amplification. Provided herein is nucleic acid encoding Rse rPTK useful as a diagnostic and in the recombinant preparation of Rse rPTK. Rse rPTK is used in the preparation and purification of antibodies thereto and in diagnostic assays.
On a identifé dans des tissus cellulaires d'origine humaine et murine la tyrosine kinase protéique récepteur (rPTK), appelée Rse. On a cloné l'ADN de codage de Rse rPTK à partir d'une bibliothèque d'ADNc d'une lignée cellulaire de carcinome de foie d'origine humaine (Hep 3B, par exemple) au moyen d'une technique d'amplification enzymatique du génome. On décrit une séquence d'acide nucléique codant Rse rPTK qui est utile dans des applications de diagnostic et dans la préparation par recombinaison de Rse rPTK. On utilise Rse rPTK dans la préparation et la purification d'anticorps dirigés contre Rse rPTK et dans des dosages de diagnostic.
Godowski Paul J.
Mark Melanie R.
Scadden David T.
Dennison Associates
Genentech Inc.
New England Deaconess Hospital
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