Proteins recruited by virus during infection of plants and...

Details Proteins recruited by virus during infection of plants and... Proteins recruited by virus during infection of plants and...

C - Chemistry, Metallurgy

07

K

C07K 14/415 (2006.01)

Patent

CA 2663827

The invention relates to a method for isolating and identifying proteins from protein- virus complexes and comprises: -the gel exclusion chromatography of virus-protein complexes extracted from infected plants or such as obtained by adding a purified virus to soluble proteins extracted from non infected plants, -SDS-PAGE of the proteins -analysis by NanoLC-MS/MS after tryptic digestion and protein identification.

La présente invention concerne un procédé visant à isoler et à identifier des protéines à partir de complexes protéine-virus, lequel procédé comprend : la chromatographie d'exclusion sur gel de complexes virus-protéine extraits de plantes infectées ou obtenus par exemple par ajout d'un virus purifié à des protéines solubles extraites de plantes non infectées; l'électrophorèse SDS-PAGE des protéines; puis l'analyse par NanoLC-MS/MS après digestion tryptique et identification des protéines.

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