Proteoliposomes containing an integral membrane protein...

Details Proteoliposomes containing an integral membrane protein... Proteoliposomes containing an integral membrane protein...

A - Human Necessities

61

K

A61K 9/127 (2006.01)

Patent

CA 2395882

A stable proteoliposome containing an integral membrane protein in a lipid membrane around an elliptoid or spherical shape. The shape preferably contains an attractant such as streptavidin or avidin and the lipid membrane contains a moiety that binds to the attractant such as biotin. The integral membrane protein is bound to a ligand which is anchored in the shape. Methods for making the proteoliposomes are provided. Kits for making the proteoliposome are described, as are the uses of the proteoliposome.

La présente invention concerne un protéoliposome stable qui contient une protéine intrinsèque située dans une membrane lipidique autour d'une forme elliptoïde ou sphérique. La forme contient de préférence un attractif tel que la streptavidine ou l'avidine et la membrane lipidique contient une fraction qui se lie à l'attractif telle que la biotine. La protéine intrinsèque est liée à un ligand qui est ancré dans la forme. Des procédés de préparation des protéoliposomes sont décrits ainsi que des trousses de préparation du protéoliposome et que les utilisations dudit protéoliposome.

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