Proteorhodopsin mutants with improved optical characteristics

Details Proteorhodopsin mutants with improved optical characteristics Proteorhodopsin mutants with improved optical characteristics

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C07H 21/04 (2006.01) C07K 1/107 (2006.01) C07K 14/195 (2006.01) C07K 14/705 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2506987

The present invention is directed to a protoeorhodopsin mutant having improved optical characteristics. One improved optical characteristic is having a lower pH(pKrh) at which equal concentrations of the acidic and basic spectral form of the proteorrhodopsin molecules are present. Another improved optical characteristic is having a smaller difference in maximum absorption wavelength between the basic and the acidic form. The mutant comprises a mutation in a conserved amino acid residue of a proteorhodopdin variant, which auses spectral shifts. A preferred mutation site is a conserved histidine residue at amino acid position 75 of Bac31A8, or position 77 of Hot75m1, or its equivalent position of a proteorhodopsin variant. Another preferred mutation site is a conserved arginine residue at amino acid position 94 of Bac31A8, or position 96 of Hot75m1, or its equivalent position of a proteorhodopsin variant.

L'invention concerne un mutant de la protéorhodopsine doté de caractéristiques optiques améliorées. Une caractéristique optique améliorée repose sur un pH inférieur (p<i>K</i>¿rh?), auquel sont présentes des concentrations égales de forme spectrale acide et basique des molécules de la protéorhodopsine. Une autre caractéristique optique améliorée consiste à présenter une différence plus petite de la longueur d'onde d'absorption maximale entre la forme basique et acide. Ledit mutant comprend une mutation dans un résidu d'acide aminé conservé d'un variant de la protéorhodopsine qui provoque des déplacements spectraux. Un site de mutation préféré est un résidu d'histidine conservé au niveau de la position 75 de l'acide aminé de Bac31A8 ou de la position 77 de Hot75m1 ou de sa position équivalente d'un variant de la protéorhodopsine. Un autre site de mutation préféré est un résidu d'arginine conservé au niveau de la position 94 de l'acide aminé de Bac31A8 ou de la position 96 de Hot75m1 ou de sa position équivalente d'un variant de la protéorhodopsine.

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