Prrsv recombinant proteins, diagnostic kits and vaccines...

Details Prrsv recombinant proteins, diagnostic kits and vaccines... Prrsv recombinant proteins, diagnostic kits and vaccines...

C - Chemistry, Metallurgy

12

N

C12N 15/40 (2006.01) A61K 39/12 (2006.01) A61K 39/295 (2006.01) A61K 39/42 (2006.01) C07K 14/08 (2006.01) C12N 7/01 (2006.01) C12N 15/86 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2167080

Recombinant proteins of the causative virus of porcine reproductive and respiratory syndrome (PRRS), corresponding to ORFs 2 to 7 of the PRRSV Spanish isolate (PRRS-Olot), have been produced in baculovirus expression system using Sf9 cell cultures as a permissive host. These recombinant proteins are suitable for the formulation of vaccines capable of efficaciously protecting porcine livestock from PRRS and for the preparation of diagnostic kits adequate for detection of anti-PRRSV antibodies as well as of PRRSV in a pig biological sample. This invention is of interest to Veterinary Medicine.

On a pu produire des protéines recombinantes du virus provoquant le syndrome respiratoire et reproductif porcin (PRRSV), qui correspondent aux ORFs 2-7 du PRRSV isolé en Espagne, (PRRS-Olot), dans un système d'expression de baculovirus recombinants multipliés dans une culture de cellules d'un hôte permissif. Ces protéines recombinantes sont appropriées pour formuler des vaccins pouvant protéger efficacement le bétail porcin contre le virus du PRRS et également pour préparer des kits de diagnostic adéquats pour la détection de la présence d'anticorps qui reconnaissent le PRRSV et également la présence du PRRSV dans un échantillon biologique porcin. Cette invention s'applique au domaine vétérinaire.

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