Pseudomonas exotoxins of low animal toxicity and high...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) A61K 47/48 (2006.01) C07K 14/21 (2006.01) C07K 14/495 (2006.01) C07K 14/54 (2006.01) C07K 14/73 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2082394

Improved Pseudomonas exotoxins of low animal toxicity and high cytocidal activity are described. Substitution of positively charged amino acid residues with an amino acid residue without a positive charge provides markedly changed exotoxins. Conjugation of the new exotoxins with suitable targeting agents provides cytocidal specificity for killing desired cellular entities.

L'invention se rapporte à des exotoxines de Pseudomonas améliorées ayant une toxicité animale faible et une activité cytocide élevée. On obtient des exotoxines notablement modifiées en substituant les résidus d'acides aminés positivement chargés par un reste d'acides aminés sans charge positive. La conjugaison de ces nouvelles exotoxines avec des agents de ciblage appropriés permet d'obtenir une spécificité cytocide pour la destruction d'entités cellulaires désirées.

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