Pseudotype retroviral vectors containing membrane proteins...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) A61K 35/76 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2413995

The present invention provides a retroviral vector containing a membrane protein having a hemagglutinin activity. The present inventors constructed a retroviral vector pseudotyped by the membrane protein having a hemagglutinin activity. This viral vector showed gene transfer at a high efficiency into host cells. In particular, it was established that genes can be transferred thereby at a high efficiency into cells into which genes can hardly be transferred by the conventional techniques, for example, blood cells and hematopoietic cells including hematopoietic stem cells, and mucous cells including mucosa epithelial cells. The viral vector of the present invention is highly useful as a vector for gene therapy.

Vecteur de rétrovirus contenant une protéine de membrane possédant une activité d'hémagglutinine. L'utilisation de cette protéine de membrane possédant une activité d'hémagglutinine, permet de construire le vecteur de rétrovirus du pseudo-type. Ce vecteur viral présente une efficacité élevée de transfert de gène dans des cellules hôtes. L'invention permet de transférer des gènes de façon extrêmement efficace dans des cellules dans lesquelles il était difficile d'introduire des gènes au moyen des techniques classiques, par exemple, des cellules sanguines et des cellules hématopoïétiques, y compris des cellules souche hématopoïétiques et des cellules muqueuses, y compris des cellules muqueuses épithéliales. Ce vecteur viral est extrêmement utile en tant que vecteur de thérapie génique.

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