Psychrophilic protease and psychrophilic bacteria

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/52 (2006.01) C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12N 9/48 (2006.01) C12P 21/02 (2006.01) C12P 21/04 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2211938

A novel psychrophilic protease and a microorganism having the psychrophilic protease producing ability are disclosed. The protease acts on and decomposes casein and dimethylcasein but not on ribonuclease, has an optimal temperature of about 40 ~C. Under the condition of storage at pH 7 for 1 hour, it is inactivated scarcely at a temperature up to 30 ~C, but at 40 ~C it loses about 40 % of the activity. At 50 ~C, the protease is rapidly inactivated so that the activity is completely abolished in about 15 minutes. Flavobacterium balustinum having the protease producing ability is also disclosed.

Nouvelle protéase psychrophile et micro-organisme ayant la capacité de produire ladite protéase. La protéase agit sur la caséine et la diméthylcaséine et la décompose, sans agir sur la ribonucléase, et présente une température optimale d'environ 40 ·C. Dans des conditions de stockage à un pH 7 pendant 1 heure, elle est rarement inactivée à une température allant jusqu'à 30 ·C et perd environ 40 % de son activité à 40 ·C. A 50 ·C, la protéase est rapidement inactivée et totalement inactivée en approximativement 15 minutes. Flavobacterium balustinum qui est capable de produire ladite protéase est également décrit.

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