Pteridine nucleotide analogs

C - Chemistry – Metallurgy – 07 – H

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C07H 19/22 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C07H 19/06 (2006.01)

Patent

CA 2343134

The invention provides pteridine nucleotides of formula (I) which are highly fluorescent and which can be used in the chemical synthesis of fluorescent oligonucleotide. The invention further provides for fluorescent oligonucleotide comprising one or more pteridine nucleotides. In addition the invention provides for pteridine nucleotide triphosphates which may be used as the constituent monomers in DNA amplification procedures. The pteridine nucleotides are more stable and possess higher quantum yields than structurally similar pteridine nucleotides.

L'invention concerne des nucléotides de ptéridine de la formule (I), très fluorescents, qui peuvent être utilisés dans la synthèse chimique d'oligonucléotides fluorescents. L'invention concerne également des oligonucléotides fluorescents comprenant un ou plusieurs nucléotides de ptéridine. Elle concerne en outre des triphosphates de nucléotide de ptéridine pouvant être utilisés comme monomères constitutifs dans des procédés d'amplification de l'ADN. Ces nucléotides de ptéridine sont plus stables et donnent des rendements quantiques plus élevés que ceux de nucléotides de ptéridine présentant une structure similaire.

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