C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/44 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01)
Patent
CA 2663948
The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of ~wild-type~ molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.
La présente invention concerne des variantes de la pullulanase peptidique enzymatique, les séquences géniques codant pour ces nouveaux peptides, des vecteurs d'expression comprenant ces séquences géniques, ainsi que des organismes exprimant les nouvelles variantes de pullulanase. Les nouvelles variantes de pullulanase de l'invention ont été élaborées empiriquement par utilisation de procédures d'optimisation de codons, troncation sélective de molécules "du type sauvage", et par remplacement de radicaux d'acides aminés sélectionnés. L'invention a également pour objet l'utilisation de ces nouveaux peptides de pullulanase dans l'industrie textile, l'industrie de la fermentation, l'industrie alimentaire et d'autres industries.
England George
Kolkman Marc
Miller Brian S.
Vroemen Casper
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor Division Danisco Us Inc.
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