C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/08 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2242680
The basement membrane (BM) underlying the epithelium of the intestine is generally believed to be of both epithelial and mesenchymal origin but the exact contribution of each tissue has not been directly examined in the human. In this study, we have used a newly described procedure to dissociate the human intestine into pure epithelial and corresponding mesenchymal fractions. Northern blot and RT-PCR analyses of the fractions for the presence of transcripts encoding extracellular matrix molecules revealed that the epithelium produces the formal BM molecules such as the .alpha.1, .alpha.2, and .beta.1 chains of laminin-1 and laminin-2 and the .alpha.5(IV) and .alpha.6(IV) chains of collagen as well as fibronectin, a BM- associated molecule. Interestingly, the .alpha.1 (IV) chain of collagen, which associates with the .alpha.2(IV) chain to form the main BM collagen network, as well as tenascin-C and decorin, two BM associated molecules, were found to be exclusively of mesenchymal origin. Taken together, these data support the concept that in the human, as in experimental animals, the intestinal BM is comprised of components produced from both the epithelium and the mesenchyme.
Beaulieu Jean-Francois
Goudreau Gage Dubuc
Universite de Sherbrooke
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