Purification and activation of proteins from insoluble...

C - Chemistry – Metallurgy – 07 – K

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530/3.2

C07K 14/61 (2006.01) C07K 1/36 (2006.01)

Patent

CA 1267496

ABSTRACT OF THE DISCLOSURE Purification and activation of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies can be effected by solubilizing the inclusion bodies in SDS; dialyzing or refrigerating the protein solution to remove a portion of the SDS; chromatographing the protein solution on an ion- retardation resin to remove the remaining SDS from the protein; and chromatographing the protein solution thus obtained on an anion-exchange resin.

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