Purification and detection processes using reversible...

Details Purification and detection processes using reversible... Purification and detection processes using reversible...

G - Physics

01

N

G01N 27/447 (2006.01) B01D 57/02 (2006.01)

Patent

CA 2322975

An affinity electrophoresis process is described, in which the direction of electrophoresis is varied in a cyclical manner while synchronously changing one or more property of the electrophoretic medium between two states, said states being characterized as favoring or disfavoring specific reversible binding of sample analytes to affinity ligands which are immobilized within the medium. The resulting process enables extremely efficient and convenient separation of the specific analytes for detection or purification, using simple materials and apparatus.

L'invention concerne un procédé d'électrophorèse d'affinité dans lequel on fait varier le sens de l'électrophorèse de manière cyclique tout en changeant de manière synchronisée une ou plusieurs propriétés du milieu électrophorétique entre deux états, lesdits états étant caractérisés comme favorisant ou défavorisant la liaison réversible spécifique de substances à analyser d'échantillons à des ligands d'affinité immobilisés dans le milieu. Le procédé obtenu permet une séparation extrêmement efficace et pratique des substances à analyser spécifiques à détecter ou à purifier, avec des matériels et un appareil simples.

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