C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/88 (2006.01) C07K 16/40 (2006.01) C12P 19/26 (2006.01) G01N 33/573 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2150263
A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Monoclonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
Est présenté ici un mécanisme unique et reproductible permettant de purifier simultanément les trois lyases hépariniques de F. heparinum jusqu'à obtention d'une homogénéité apparente. Les propriétés cinétiques des lyases hépariniques ont été déterminées, ainsi que les conditions permettant d'optimiser leur activité et leur stabilité. On décrit également les anticorps monoclonaux dirigés contre les trois héparinases, qui facilitent la détection, l'isolement et la caractérisation de celles-ci.
Cooney Charles L.
Langer Robert S.
Linhardt Robert J.
Lohse Daniel L.
Sasisekharan Ramnath
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Massachusetts Institute Of Technology
University Of Iowa Research Foundation
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