C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 1/36 (2006.01) C07K 7/14 (2006.01) C07K 14/245 (2006.01) C07K 14/47 (2006.01) C07K 14/585 (2006.01) C07K 14/595 (2006.01) C12N 9/88 (2006.01) C12N 15/11 (2006.01) C12N 15/60 (2006.01) C12P 21/06 (2006.01)
Patent
CA 2087261
The present invention is directed to microbial and higher organism methods for expression and ligand immobilized affini- ty purification of a product peptide. The biological compositions of the invention which are produced by these methods include or are designed to express, a protein purification construct having three tandem, coupled segments composed of a binding pro- tein, an interconnecting peptide and a variable fused polypeptide which incorporates the product peptide in one or more copies. According to the method of the invention, the following steps will allow production of the product peptide: (a) host cell or host organism expression regulated by the recombinant expression vector carrying the recombinant gene coding for the protein purifi- cation construct; (b) purification of the protein purification construct by a ligand immobilized affinity separation technique; and (c) enzymatic or chemical cleavage of the protein purification construct to produce the product peptide.
Coolidge Thomas R.
Schuster Sheldon M.
Stout Jay
Van Heeke Gino
Wagner Fred
Bionebraska Inc.
Goudreau Gage Dubuc
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