C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/81 (2006.01) C07K 1/18 (2006.01) A61K 38/57 (2006.01) C12N 7/04 (2006.01)
Patent
CA 2156007
Cation chromatography with solutions at pH less than 6.0 and low ionic strength can be utilized to purify human .alpha.-1. proteinase inhibitor (.alpha.-1 PI) from biological fluids including plasma and plasma fractions. The cation chromatography takes advantage of the fact that active .alpha.-1 PI does not bind to the cation column under these conditions but other proteins, including denatured .alpha.-1 PI and albumin, do. The effect is that active .alpha.-1 PI flows through the chromatography column leaving the contaminating proteins behind. Recovery of .alpha.-1 PI is high and improvement of purity is dramatic.
On peut utiliser la chromatographie cationique de solutions ayant un pH de moins de 6,0 et une faible force ionique pour purifier l'inhibiteur de l'alpha-1 protéinase humain (.alpha.-1 PI) des fluides biologiques, notamment le plasma et les fractions plasmatiques. La chromatographie cationique profite du fait que l'alpha.-1 PI actif ne se lie pas à l'appareil échangeur de cations sous ces conditions, contrairement à d'autres protéines, comme l'alpha.-1 PI dénaturé et l'albumine. En conséquence, l'alpha.-1 PI actif passe à travers l'appareil échangeur de cations, en laissant derrière lui les protéines contaminantes. Le taux de récupération de l'alpha.-1 PI est élevé, et son degré de pureté est considérablement amélioré.
Chen Sharon X.
Lebing Wytold R.
Chen Sharon X.
Lebing Wytold R.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Talecris Biotherapeutics Inc.
LandOfFree
Purification of alpha-1 proteinase inhibitor using novel... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of alpha-1 proteinase inhibitor using novel..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of alpha-1 proteinase inhibitor using novel... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1589584