C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/00 (2006.01) A61K 38/46 (2006.01) C12N 1/08 (2006.01) C12N 1/12 (2006.01) C12N 1/14 (2006.01) C12N 1/16 (2006.01) C12N 1/20 (2006.01) C12N 5/02 (2006.01) C12N 9/10 (2006.01) C12N 9/22 (2006.01)
Patent
CA 2325575
The invention relates to host cells and methods of preparing a substantially RNA-free cellular component, comprising culturing cells producing the cellular component in a medium and lysing the cells to produce a cell lysate, wherein the cell lysate contains the cellular component and sufficient RNase activity to degrade substantially all of the RNA molecules present in the cell lysate. The invention also relates to substantially RNA-free cellular components.
La présente invention concerne des cellules hôtes et des procédés permettant de préparer un composant sensiblement exempt d'ARN. Ce procédé consiste à cultiver dans un milieu des cellules produisant le composant cellulaire, puis à lyser les cellules de façon à produire un lysat cellulaire. En l'occurrence, ce lysat cellulaire qui contient le composant cellulaire fait preuve d'une activité ribonucléase qui suffit pour dégrader sensiblement toutes les molécules d'ARN présentes dans le lysat cellulaire. L'invention concerne également des composants cellulaires sensiblement exempts d'ARN.
Hanak Julian Alexis John
Williams Steven Geraint
Cobra Therapeutics Limited
Ridout & Maybee Llp
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