Purification of d-aminoacid oxidase

Details Purification of d-aminoacid oxidase Purification of d-aminoacid oxidase

C - Chemistry, Metallurgy

12

P

195/123, 195/78

C12P 21/00 (2006.01) B01J 20/32 (2006.01) C12N 9/06 (2006.01)

Patent

CA 1050912

PURIFICATION OF D-AMINOACID OXIDASE ABSTRACT OF THE DISCLOSURE Process for the separation and purification of d-aminoacid oxidase by affinity chromatograph. The process comprises contacting a mixture of proteins containing the d-aminoacid oxidase with a mineral carrier carrying haloalkyl- silane grafts, upon which are fixed - NH - (CH2)3- NH - (CH2)2 C6H4 COOH residues. The enzyme retained by the carrier is then separated by a solution of ethyleneglycol. Use of the pure d-aminoacid oxidase for the preparation of cetonic acids.

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