Purification of hapten-carrier generated antibodies

Details Purification of hapten-carrier generated antibodies Purification of hapten-carrier generated antibodies

A - Human Necessities

61

K

A61K 39/395 (2006.01) C07K 16/06 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2101148

1174-6 PURIFICATION OF HAPTEN-CARRIER GENERATED ANTIBODIES ABSTRACT In the commonly used method to obtain antibodies to small molecules, a combination of a highly antigenic carrier, such as bovine serum albumin, and the small molecule is injected into a host animal. The recovered crude serum or plasma then contains, in addition to the desired small molecule antibodies, much larger amounts of carrier-induced antibodies. These unwanted antibodies are efficiently removed from the crude serum or plasma, by contacting the crude serum or plasma with the carrier material in an immobilized high surface area form. Rapid and efficient anticarrier antibody removal results, with minimal loss of both desired antibody, and desired antibody activity.

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