Purification of hemoglobin and methemoglobin by bioselective...

Details Purification of hemoglobin and methemoglobin by bioselective... Purification of hemoglobin and methemoglobin by bioselective...

C - Chemistry, Metallurgy

07

K

530/15.22, 530/3

C07K 14/805 (2006.01) C07K 1/18 (2006.01)

Patent

CA 1338244

A method for purifying hemoglobin, mixed with other proteins is described, wherein the mixture is contacted with an ion exchange matrix so that the hemoglobin is adsorbed to it. The matrix is washed to remove unadsorbed components, and the hemoglobin is then selectively eluted using a ligand which specifically binds the hemoglobin causing it to desorb from the matrix. The method can also be used to effect reduction of methemoglobin to hemoglobin on an ion exchange matrix followed by selective elution to achieve purification. The method can also be used to purify methemoglobin.

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