A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
167/114
A61K 35/18 (2006.01) C07K 14/805 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1244349
ABSTRACT The invention disclosed relates to a method for the purification of hemoglobin by the technique of affinity chromatography. Contrary to current belief, the binding of oxygen and selected polyanions to hemoglobin is not mutually exclusive. The novel method comprises immobilizing a polyanion which specifically binds hemoglobin via its polyanion binding site, on a chromatographic gel and passing the hemoglobin containing solution or mixture through the gel. The hemoglobin is thus retained in the gel, while impurities are eluted from the gel. The novel method is also applicable to the separation of unmodified hemoglobin from a liquid reaction mixture containing modified and unmodified hemoglobin.
485392
Anderson J. Wayne
As Represented By The Ministe R. Of National Defence Her Majesty The Queen In Right Of Canada
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