Purification of hepatitis pre-s antigens by polymerized...

Details Purification of hepatitis pre-s antigens by polymerized... Purification of hepatitis pre-s antigens by polymerized...

A - Human Necessities

61

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167/130

A61K 39/29 (2006.01) C07K 14/02 (2006.01)

Patent

CA 1282696

5078P/1116A 17362 TITLE OF THE INVENTION PURIFICATION OF HEPATITIS PRE-S ANTIGENS BY POLYMERIZED SERUM ALBUMIN AFFINITY BINDING ABSTRACT OF THE DISCLOSURE Recombinant pre-S-HBsAg is purified by a rapid and efficient two step chromatographic process. Yeast cells expressing recombinant pre-S-HBsAg are disrupted, the cell contents are clarified and separated by polymerized human serum albumin affinity chromatography. The pre-S-HBsAg is further purified by hydrophobic interaction chromatography using butyl agarose. This process results in pre-S-HBsAg that is greater than 90% pure.

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