Purification of her-2 variants

Details Purification of her-2 variants Purification of her-2 variants

C - Chemistry, Metallurgy

07

K

C07K 14/47 (2006.01) C07K 1/16 (2006.01)

Patent

CA 2530233

The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.

L'invention concerne un nouveau procédé de purification de protéines de la famille EGFR obtenues à partir de cultures de cellules d'insectes. Le procédé comprend les étapes de a) défiltration et échange de milieu de culture avec un tampon, b) chromatographie d'affinité d'ions métalliques immobilisés (IMAC), c) chromatographie d'exclusion (SEC), et d) chromatographie d'échange d'anions (AIE). Le procédé comprend également un variant immunogène de protéine HER-2 pour lequel le procédé de purification a été spécialement conçu, ainsi que des moyens de préparation dudit variant.

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