C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/00 (2006.01) C07K 1/22 (2006.01) G01N 33/533 (2006.01)
Patent
CA 2759502
Disclosed are methods of purifying an antibody-agent conjugate from unconjugated antibody and unconjugated agent. In some embodiments, the method comprises application of conditions to allow binding of a conjugate, an unconjugated antibody, and an unconjugated agent from a mixture of said components to a mixed mode chromatography support. The support is then contacted with a buffer such that the unconjugated antibody and unconjugated agent are substantially removed from the support while at least a majority of the conjugate remains bound to the support. Finally, the conjugate is eluted from the support, thereby purifying the antibody-agent conjugate from unconjugated antibody and unconjugated agent.
La présente invention concerne des procédés de purification d'un conjugué agent - anticorps à partir d'un anticorps non conjugué et d'un agent non conjugué. Dans certains modes de réalisation, le procédé comprend l'application de conditions permettant de lier un conjugué, d'un anticorps non conjugué, et d'un agent non conjugué à partir d'un mélange desdits composants sur un support chromatographique en mode mixte. Le support est ensuite mis en contact avec une solution tampon de sorte que l'anticorps non conjugué et l'agent non conjugué soient pratiquement éliminés du support tandis qu'une majorité du conjugué reste lié au support. Pour finir, le conjugué est élué à partir du support, purifiant de ce fait le conjugué agent - anticorps de l'anticorps non conjugué et de l'agent non conjugué.
Bio-Rad Laboratories Inc.
Fetherstonhaugh & Co.
LandOfFree
Purification of immunoconjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of immunoconjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of immunoconjugates will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1409959