Purification of monomeric interferon

Details Purification of monomeric interferon Purification of monomeric interferon

C - Chemistry, Metallurgy

07

K

530/3.18, 530/3.

C07K 14/56 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1329307

ABSTRACT Fast moving monomers of alpha-2 interferon ("IFN- alpha-2") are purified from slow moving monomers and oligomers of interferon and from noninterferon proteins using a combination of immunoaffinity chromatography, reverse phase HPLC, cation exchange chromatography, and gel filtration. Two fast monomer forms, FMM1 and FMM2, are further distinguished on the basis of charge and hydrophobicity and FMM2 is obtained in essentially homogeneous form with respect to size, hydrophobicity and charge.

579397

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