Purification of neurotrophins

Details Purification of neurotrophins Purification of neurotrophins

C - Chemistry, Metallurgy

07

K

C07K 14/475 (2006.01) C07K 1/20 (2006.01) C07K 14/48 (2006.01)

Patent

CA 2268747

Methods are provided for large scale purification of neurotrophins, including mature NGF, suitable for clinical use. The methods provide means to separate neurotrophins from various less desirable misprocessed, misfolded, size, glycosylated, or charge forms. Compositions of neurotrophins, including mature NGF, substantially free of these variants are also provided.

Procédés de purification à grande échelle de neurotrophines y compris du facteur de croissance nerveuse (FCN) mature adoptés à l'usage clinique. Les procédés utilisant des moyens permettant de séparer des neurotrophines de diverses formes moins désirables, mal traitées, mal pliées, glycosylées ou n'ayant pas la bonne taille ou charge. On décrit également des compositions de neurotrophines comprenant le FCN mature, qui sont sensiblement dépourvues de ces variants.

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