Purification of nucleotide sequences suitable for expression...

Details Purification of nucleotide sequences suitable for expression... Purification of nucleotide sequences suitable for expression...

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/00 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01)

Patent

CA 1157406

ABSTRACT OF THE DISCLOSURE A purification procedure for cDNA of desired nucleotide sequence complementary to an individual mRNA species is disclosed wherein restriction endonuclease cleavage of cDNA transcribed from a complex mixture of mRNA is utilized. In this way, extensive purification of RNA is not required but rather the method makes use of transcription of RNA into cDNA, the sequence specific fragmentation of this cDNA with one or two restriction endonucleases and fractionation of the cDNA restriction fragments on the basis of their length.

303860

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