Purification of pegylated polypeptides

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/505 (2006.01) A61K 38/18 (2006.01) A61K 47/48 (2006.01) C07K 1/18 (2006.01)

Patent

CA 2692612

The current invention comprises a method for the purification of mono- PEGylated erythropoietin comprising two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chro-matography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.

La présente invention concerne un procédé de purification d'érythropoïétine mono-polyéthylène-glycolée. Ce procédé fait intervenir deux étapes de chromatographie d'échange de cations, chaque étape utilisant le même matériau d'échange de cations. L'invention concerne également un procédé de production d'érythropoïétine mono-polyéthylène-glycolée sous une forme sensiblement homogène.

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