Purification of pertussis antigens

Details Purification of pertussis antigens Purification of pertussis antigens

A - Human Necessities

61

K

167/135

A61K 39/10 (2006.01) C07K 14/235 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1280693

ABSTRACT PURIFICATION OF PERTUSSIS ANTIGENS A process is provided for the production of lymphocytosis promoting factor (LPF), filamentous haemagglutinin (FHA) and at least one fimbrial agglutinogen from a liquidculture of Bordetella pertussis, which comprises the steps of (a) separating the culture into cellular and supernatant fractions, (b) concentrating the supernatant fraction, (c) fractionating the concentrated supernatant fraction to isolate LPF and FHA containing fractions, and (d) isolating at least one fimbrial agglutinogen from the cellular fraction. A vaccine composition may be produced by mixing so-produced LPF, FHA and fimbrial agglutinogens produced.

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