Purification of pertussis toxins

Details Purification of pertussis toxins Purification of pertussis toxins

C - Chemistry, Metallurgy

07

K

167/137, 195/78,

C07K 14/235 (2006.01) A61K 39/10 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1331134

ABSTRACT OF THE DISCLOSURE Lymphocytosis promoting factor (LPF) and filamentous hemaglutinin (FHA) are isolated from e growth medium of the Bordatella pertussis organism and purified by selecting adsorbing the LPF and FHA on a selective adsorbing medium, such as filter aids or gel filtration media, at low ionic strength and subsequently removing the adsorbed LPF and FHA at using an aqueous medium of high ionic strength, either simultaneously or sequentially. Prior to desorbtion of the LPF and FHA, the adsorbing medium may be contacted with an aqueous solution of a non-ionic detergent, which enables the LPF and FHA subsequently desorbed to be substantially free from contamination by lipopolysaccharides (LPS). The LPF and FHA may by further purified on hydroxyapatite. The LPF and FHA may be detoxified separately or together by contacting with a cross-linking agent, such as glutaraldehyde or formaldehyde, in the presence of an anti-aggregation agent. The resulting purified and detoxified LPF and FHA may be used to formulate a vaccine against pertussis.

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