Purification of pichia produced lipophilic proteins

Details Purification of pichia produced lipophilic proteins Purification of pichia produced lipophilic proteins

C - Chemistry, Metallurgy

12

P

195/73, 530/3.02

C12P 21/02 (2006.01) C07K 1/00 (2006.01) C07K 14/02 (2006.01) C12N 1/06 (2006.01) C12N 9/04 (2006.01)

Patent

CA 1277272

Abstract Method for the selective extraction of desired lipophilic proteins from transformed cells of the genus Pichia by cell lysis in the presence of chaotrophic salts is disclosed. The total protein extracted under the invention cell lysis conditions is reduced while the recovery of desired lipophilic proteins remains relatively constant, thereby producing a cell extract with enhanced concentration of the desired lipophilic protein relative to a control cell extract.

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