C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2262820
A method for purifying plasmid DNA through precipitation with a condensating agent and column chromatography is provided. A short chain polymeric alcohol, preferably polyethylene glycol, or another DNA condensation agent, is added to a DNA sample prior to column chromatography. The short chain polymeric alcohol or condensation agent promotes improved isolation of plasmid DNA and may be used for large scale purification, particularly for manufacturing plasmid DNA as a biopharmaceutical.
La présente invention concerne un procédé de purification de l'ADN plasmidique par précipitation avec un agent de condensation et chromatographie sur colonne. Le procédé consiste à ajouter à l'échantillon d'ADN, préalablement à la chromatographie sur colonne, un alcool polymère à chaîne courte, de préférence du polyéthylène glycol, ou un autre agent de condensation de l'ADN. Cet alcool polymère à chaîne courte ou cet agent de condensation, qui favorise une meilleure séparation de l'ADN plasmidique, convient à des opérations de purification de grande envergure, en particulier la confection d'ADN plasmidique considéré comme produit biopharmaceutique.
Budahazi Greg
Horn Nancy
Marquet Magda
Mbm Intellectual Property Law Llp
Vical Incorporated
LandOfFree
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