Purification of proteins employing ctap-iii fusions

Details Purification of proteins employing ctap-iii fusions Purification of proteins employing ctap-iii fusions

C - Chemistry, Metallurgy

12

N

530/13, 195/1.29

C12N 15/62 (2006.01) C07K 14/575 (2006.01) C07K 14/745 (2006.01) C07K 14/78 (2006.01) C07K 14/815 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2016018

- 67 - PURIFICATION OF PROTEINS EMPLOYING CTAP-III FUSIONS Abstract of the Invention The present invention provides a process for the recovery of heterologous proteins from CTAP-III fusion proteins comprising expressing a fusion protein having a first amino acid sequence, a second amino acid sequence, and a selectable site which may be cleaved to provide first and second polypeptide fragments, respectively, wherein the first amino acid fragment is homologous to CTAP-III, and the first and second fragments have different pI values; cleaving the fusion protein to provide the first and second fragments; and separating the first and second fragments by ion exchange chromatography.

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