Purification of proteins with cationic surfactant

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/30 (2006.01) C07K 1/113 (2006.01) C07K 14/195 (2006.01) C12N 9/00 (2006.01) C12N 9/06 (2006.01)

Patent

CA 2611249

The subject invention provides a method for purifying a target protein from a mixture comprising the target protein and contaminating protein, comprising the steps of exposing the mixture to an effective amount of a cationic surfactant such that the contaminating protein is preferentially precipitated and recovering the target protein. Proteins purified according to the method of the invention are also provided.

L'invention concerne une méthode de purification d'une protéine cible à partir d'un mélange comprenant la protéine cible et une protéine contaminante. La méthode consiste à placer le mélange au contact d'une quantité efficace d'un tensioactif cationique de telle sorte que la protéine contaminante soit, de préférence, précipitée; puis à récupérer la protéine cible. L'invention concerne également des protéines purifiées selon ladite méthode.

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