C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/3.26
C07K 14/02 (2006.01)
Patent
CA 1304887
PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN ABSTRACT OF THE DISCLOSURE Yeast proteins hydrophobically bonded to a desired protein or polypeptide produced in a trans- formed yeast are removed by treating the antigen with a chaotropic agent and concentrating the treated antigen in a hollow fiber capillary bundle having a molecular weight cutoff greater than 50,000 daltons but less than 1,000,000 daltons. The concentrated antigen is then diafiltered using a chaotropic agent to remove substantially all remaining yeast protein.
560864
Hurni William M.
Kubek Dennis J.
Miller William J.
Rienstra Mark S.
Scolnick Edward M.
Gowling Lafleur Henderson Llp
Hurni William M.
Kubek Dennis J.
Merck & Co. Inc.
Miller William J.
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