Purification procedure of tpa from crude preparations

Details Purification procedure of tpa from crude preparations Purification procedure of tpa from crude preparations

C - Chemistry, Metallurgy

12

N

195/127, 195/39.

C12N 9/64 (2006.01) C12N 9/72 (2006.01) C12N 15/00 (2006.01)

Patent

CA 1314833

- 21 - ABSTRACT OF THE DISCLOSURE Crude tPA containing, as an impurity, a protein which is reactive with an anti-human tPA antibody and has a molecular weight of 110,000 + 20,000 daltons is purified by bringing the crude tPA into close contact with an affinity reagent containing an immobilized Kunitz inhibitor which is produced in seeds of Erythrina latissima and other Erythrina plants and acts as an inhibitor on trypsin, plasmin and tPA but does not act on urokinase, so that tPA is collected selectively.

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